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HomeAnalytical InstrumentsSpectrophotometer เครื่องวัดการดูดกลืนของแสงNanodrop NanoDrop Ultra Microvolume UV-Vis Spectrophotometers and Fluorometers
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NanoDrop Ultra Microvolume UV-Vis Spectrophotometers and Fluorometers

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Description

NanoDrop™ Ultra Spectrophotometers and Fluorometers

Thermo Scientific™ NanoDrop™ Ultra เครื่องตรวจจับไมโครโวลุ่มรุ่นใหม่ อีกหนึ่งขั้นของความแม่นยำและความรวดเร็วสำหรับการวิเคราะห์กรดนิวคลีอิกและโปรตีนด้วยปริมาตรตัวอย่างเพียง 1.0 μL NanoDrop Ultra ได้รับการออกแบบมาเพื่อปรับปรุงขั้นตอนการทำงานในหลากหลายสาขาทั้งด้าน genomics, proteomics, molecular biology, biotechnology, pharmaceutical,  life sciences research โดยให้ประสบการณ์ในการใช้งานที่ดีที่สุด และความแม่นยำสูงสุดในเครื่องเดียว

ด้วยเทคโนโลยี Thermo Scientific™ Acclaro™ Sample Intelligence ที่เป็นนวัตกรรมใหม่ได้ถูกสร้างขึ้นในทุกเครื่องมือเพื่อปรับปรุงความแม่นยำในการวัดและการระบุสิ่งปนเปื้อน ระบุปริมาณและคุณสมบัติของตัวอย่าง DNA, RNA และโปรตีนในไม่กี่วินาทีด้วยปริมาตรเพียง 1-2μL และรับข้อมูลสเปกตรัมเต็มรูปแบบก่อนที่คุณจะตัดสินใจใช้ตัวอย่างในการใช้งานขั้นปลาย นอกจากนี้ยังมีรุ่นที่ใช้งานร่วมกันได้กับ Cuvette เพื่อความยืดหยุ่นเพิ่มเติม

NanoDrop รุ่น NanoDrop Ultra บางรุ่นจะมีทั้งความสามารถในการดูดกลืนแสงไมโครโวลุ่ม(absorbance) และการเรืองแสง  (fluorescence)  เมื่อจับคู่กับการออกแบบที่กะทัดรัดและหน้าจอสัมผัสขนาดใหญ่ความละเอียดสูง 10.1 นิ้ว เครื่องสเปกร์ NanoDrop Ultra จึงเหมาะอย่างยิ่งสำหรับผู้ใช้ที่กำลังมองหาความยืดหยุ่นและความสะดวกสบาย ซอฟต์แวร์ Acclaro Pro ซึ่งเป็นตัวเลือกเสริมที่ใช้งานร่วมกันได้กับเครื่องมือ NanoDrop Ultra ช่วยปลดล็อกความแม่นยำที่สูงขึ้นสำหรับตัวอย่างที่มีความเข้มข้นสูงด้วยอัลกอริทึมที่ได้รับการปรับปรุง

Acclaro Sample Intelligence Technology ยกระดับความเข้าใจในคุณภาพตัวอย่าง

เป็นนวัตกรรมที่ช่วยให้ผู้ใช้เข้าใจคุณภาพของตัวอย่างได้ดียิ่งขึ้น พร้อมทั้งให้ผลการวัดเชิงปริมาณที่แม่นยำ

  •     การตรวจจับและวิเคราะห์สิ่งปนเปื้อน: เทคโนโลยีนี้ให้การประเมินความบริสุทธิ์ของตัวอย่างอย่างครอบคลุม และยังสามารถแก้ไขค่าการดูดกลืนแสงของตัวอย่างได้ ทำให้ผู้ใช้สามารถระบุ ปริมาณ และลดผลกระทบของสิ่งปนเปื้อนได้อย่างมั่นใจ นอกจากนี้ ยังสามารถแยกแยะ DNA และ RNA ของพืชและแบคทีเรียได้ ซึ่งเป็นประโยชน์สำหรับนักวิจัยและผู้เชี่ยวชาญในสาขาต่างๆ เช่น เกษตรกรรม ชีววิทยาของพืช จุลชีววิทยา และพันธุวิศวกรรม เทคโนโลยี Acclaro สามารถจดจำสารที่ไม่พึงประสงค์ได้หลายชนิด ทำให้สามารถตรวจจับความเป็นไปได้ของตัวอย่างที่ปนเปื้อนได้อีกด้วย
  • Sample integrity insight : แจ้งเตือนแบบเรียลไทม์เมื่อตรวจพบฟองอากาศในตัวอย่าง ซึ่งอาจนำไปสู่การวัดที่ไม่ถูกต้อง
  • Reliable accuracy : เทคโนโลยี Acclaro ช่วยให้มั่นใจในความสมบูรณ์ของการวัดด้วยเซ็นเซอร์ในตัวและการวิเคราะห์ภาพดิจิทัs.

วิเคราะห์ได้ทั้ง การดูดกลืนแสงไมโครโวลุ่ม(absorbance) และการเรืองแสง  (fluorescence)

ข้อมูลที่ครบถ้วนที่สุด เกี่ยวกับความเข้มข้นและคุณภาพของตัวอย่าง DNA หรือ RNA ของคุณ โดยทำการวัดทั้ง การดูดกลืนแสง (absorbance) และ การเรืองแสง (fluorescence) บนเครื่องเดียว สำหรับรุ่น NanoDrop Ultra FL และ NanoDrop UltraC FL สามารถทำการวัดทั้งการดูดกลืนแสงแบบไมโครปริมาตรและการเรืองแสงได้

  • ความไวสูง: การวัดแบบเรืองแสงมีความไวสูงกว่าการวัดปริมาณโดยอิงจากการดูดกลืนแสง สามารถตรวจวัด dsDNA หรือ RNA ได้ต่ำถึง 0.05 ng/μ
  • ระบบคำนวนตัวอย่าง : มีฟังชั่นในตัวที่สามารถคำนวณปริมาณสีย้อมและบัฟเฟอร์ที่จำเป็นสำหรับการทดลองของคุณได้

 

 

Tools to aid in 21 CFR Part 11 compliance

Optional Thermo Scientific™ SciVault™ 2 software installs on either the instrument or a companion PC and gives labs the tools they need to comply with US FDA 21 CFR Part 11.

  • Compliance made easy – SciVault 2 integrates into the NanoDrop Ultra software user interface and controls user account management, user account access, digital signatures, and audit logs.
  • On-board options – software is available to be run directly on the local instrument through on-board user account management or you can connect your instrument to your domain to use your Windows account management.
  • Centralized solution – install SciVault 2 software on a central computer to control privileges and view audit logs across multiple NanoDrop Ultra instruments in different labs.

Streamlined workflow

  • Fully integrated qPCR recipe calculator – use the built-in library of kits, or add your favorite, to let the software run the math for you. The qPCR recipe calculator suggests how to prepare the reaction based on your sample’s concentration.
  • Auto-Measure and Auto-Blank functions – multi-sample processing can be streamlined with instant measurements that occur when the pedestal arm is down. These functions can be turned ON or OFF with a single tap on the screen.

Advanced connectivity for data storage, printing and sharing

  • Export to the cloud – export data directly to popular cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
  • Direct LIMS integration – send data directly into your LIMS with the assistance of an integrated RESTful API.
  • Easy data-to-printer options – Print your results directly from the NanoDrop Ultra instrument via Wi-Fi, Ethernet or USB printer connection; print exported data from your PC using the Nanodrop Ultra PC Control software.
  • Data Your Way – customize and preview the information included in the data file prior to export to ensure it meets any downstream needs.

Accuracy beyond expectations with the Acclaro Pro Software

This optional software package includes new versions of the most popular measurement applications which are unlocked from the factory, providing ±5% Error throughout the absorbance dynamic range.

  • Accurate high concentration measurements – absorbance measurements within 5% error up to 550 Abs (10 mm equivalent) – up to 400 mg/mL IgG or 18,000 ng/μL.
  • Quick dilution-free process – take triplicate measurements on the pedestal in 2 minutes.
  • Enhanced calibration – bundled purchases get an additional calibration from the factory to calibrate the instrument for exceptional accuracy within the Acclaro Pro applications.

For research use only; not for diagnostic purposes.

NanoDrop Ultra spectrophotometers and fluorometers specifications

Category Attribute Description
Pedestal Spectrophotometric Minimum Sample Size 1 μL
Sample Number 1
Limit of Detection 1 ng/μL (dsDNA), 0.06 mg/mL (BSA)
Maximum Concentration 27,500 ng/μL (dsDNA), 820 mg/mL (BSA)
Pathlength(s) 0.030 to 1.0 mm automatically adjusting
Measurement Repeatability1 0.002 A (1.0 mm path) or 1% CV, whichever is greater
Photometric Range 0.02 – 550 Abs (10 mm equivalent)
Photometric Accuracy2 3% at 0.97 A, 302 nm, 23 ± 2C°
Measurement & Data Processing Time  ≤ 7 sec
Acclaro Pro – Photometric Accuracy 5% up to 550 Abs
Acclaro Pro – Measurement & Data Processing Time  ≤ 30 sec
Cuvette Spectrophotometric Limit of Detection 0.2 ng/μL (dsDNA), 0.006 mg/mL (BSA)
Maximum Concentration 75 ng/μL (dsDNA), 2 mg/mL (BSA)
Photometric Range 0.004 – 1.5 Abs
Pathlength(s) 10 mm, 5 mm, 2 mm, 1 mm
Beam Height (Z-Height) 8.5 mm
Temperature Control 37.0 °C ± 0.5 °C
Strirring 9 speeds
Absorbance-Based Optics Light Source Xenon flash lamp
Detector Type 2048-element CMOS linear image sensor
Wavelength Range 190–850 nm
Wavelength Accuracy ±1 nm
Spectral Resolution ≤1.8 nm (FWHM at Hg 254 nm)
Pedestal Fluorescence Minimum Sample Size 2 μL
Limit of Detection   Assay Specific3
Maximum Concentration
Measurement & Data Processing Time ≤ 20 sec for Blue LED; ≤ 40 sec for Red LED
Fluoresence-Based Optics LEDs Blue (max ~470 nm) and Red (max ~635 nm)
Excitation Filters Blue (430-495 nm) and Red (600-645 nm)
Characteristics Footprint (W × D × H) 32 × 18 × 28 cm (12.6 × 7.1 × 11.0 in.)
Weight 4.1 kg
Sample Pedestal 303 stainless steel and quartz fiber
Cuvette Position Optional (with stirring) (NanoDrop UltraC and NanoDrop UltraC FL only)
Operating Voltage 12 V (DC)
Power Consumption 7W at Idle; 15W at Working Conditions
Instrument Control Built-in touchscreen or PC control with free PC software
21 CFR Part 11 Compliance Yes, optional software for use on either PC or local instrument
System Applications Support Nucleic Acid A260, A260/A280, A260/A230, Labeled Nucleic Acids, Microarray, dsDNA (Pro), ssDNA (Pro), RNA (Pro), Oligo DNA/Oligo RNA (Pro), RNA/DNA Deconvolution,  Custom Factor (Pro); Protein A280 (Pro) and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Proteins & Labels, dsDNA Fluorescence, RNA Fluorescence, Fluorometer. Protein Editor; Dye Editor, Kit Editor, OD600, Kinetics, UV-Vis, and Custom Methods
Language Support Chinese, French, German, Japanese, Korean, Polish, Spanish, English, Italian
Accessory Support DYMO® LabelWriter® 550 Turbo printer, Bluetooth keyboard, mouse, and barcode reader
  Software Compatibility Standalone, local control software
NanoDrop Ultra PC control (free download)
Thermo Scientific SciVault 2 software (purchase separately)
PC Software: Windows® 10/11 Professional (64 bit)
Acclaro Pro software (purchase seperately)
Connectivity 2x USB-A Ports, 1x USB-C Port, Ethernet, Bluetooth, Wi-Fi
  Advanced Connectivity Google Drive
Microsoft OneDrive
Thermo Fisher Connect Platform
Shipping & Delivery
Instrument

Fast, accurate DNA, RNA, and protein quantification, one sample at a time

Thermo Scientific NanoDrop Ultra Spectrophotometers and Fluorometers are compact, stand-alone UV-visible spectrophotometers (select models also function as fluorometers) for measurement of highly concentrated samples across a wide variety of analytes without the need for dilution. A glove-compatible touchscreen offers easy access to preloaded software applications and enables purity and concentration assessments in seconds. Built-in Acclaro Sample Intelligence Technology helps identify and correct for the contaminants present. Make informed decisions on sample quality and improve your success in downstream applications with NanoDrop technology.   NanoDrop Ultra instruments introduce a suite of innovative features designed to enhance your laboratory workflow. Key highlights include an integrated qPCR calculator that precisely determines the sample volume required for downstream applications, and the optional Acclaro Pro software, which incorporates an advanced algorithm for highly accurate quantification of concentrated samples. Additionally, a diagnostic scheduler alerts you when a diagnostic test is due, ensuring consistent performance. These, along with numerous other cutting-edge features, make NanoDrop Ultra instruments an indispensable tool for modern laboratories.
Choose the NanoDrop Ultra instrument for pedestal-only absorbance sampling, NanoDrop UltraC instrument for pedestal or cuvette absorbance sampling, NanoDrop Ultra FL instrument for pedestal-only absorbance and fluorescence sampling, or the NanoDrop UltraC FL instrument for pedestal absorbance and fluorescence sampling and cuvette absorbance sampling.

NanoDrop Ultra spectrophotometers and fluorometers features

Our flagship NanoDrop Ultra spectrophotometers and fluorometers share common features with other members of the NanoDrop family:

  • Pioneering pedestal technology Simply pipet a drop of your DNA, RNA, or protein sample on the pedestal and pull down the arm; results appear in seconds
  • Advanced sample intelligence By analyzing absorbance across the full spectrum, Acclaro Sample Intelligence software can identify contaminants and correct quantitation results before you use them in downstream applications
  • Rapid time to results Results appear within seconds of initiating a measurement, and Auto-Blank and Auto-Measure settings can further streamline your workflow
  • Streamlined multi-sample processing With instant Auto-Measure and Auto-Blank capabilities
  • Tiny sample sizes Requires only 1–2 µL of sample without dilution, even for highly concentrated samples
  • Differentiates DNA from RNAAcclaro software uses full-spectrum data to tease apart mammalian DNA from RNA and calculate corrected nucleic acid concentrations
  • Your dependable lab partner Since their introduction in 2001, NanoDrop spectrophotometers have become indispensable in many labs, accumulating over 55,000 scientific citations
In addition, the NanoDrop Ultra spectrophotometers and fluorometers offer indispensable features that make it a reliable, easy-to-use laboratory workhorse for many sample and assay types, including regulated environments. To learn more, see the sections below.
  • Expanded dynamic range Measure from 1 ng/µL up to 27,500 ng/µL dsDNA or 0.02 mg/mL to 400 mg/mL IgG without dilution.
 
  • Optional Acclaro Pro software for accurate high concentration measurements With the use of the optional Acclaro Pro software obtain absorbance measurements within 5% error up to 550 Abs (400 mg/mL IgG or 22,000 ng/µL ssDNA).
  • Diagnostic Scheduler Use the built-in Diagnostic Schedule application to alert you when a diagnostic test is overdue, ensuring consistent performance of the instrument.
  • Cuvette option Choose the NanoDrop Ultra or Ultra FL instrument for sample pedestal only or the NanoDrop UltraC or UltraC FL instrument for both pedestal and cuvette to expand the limit of detection to 0.2 ng/µL dsDNA or 0.006 mg/mL IgG.
  • Advanced cloud connectivity Export data directly to popular Cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
  • Optional on-board SciVault 2 software for compliance On-board Thermo Scientific SciVault 2 Software aids compliance with FDA 21 CFR Part 11 electronic recordkeeping regulations with subsequent seamless integration into your LIMS environment using RESTful API directly on the local instrument. SciVault 2 software can also be installed on a connected computer if desired.
  • All-in-one absorbance and fluorescence analysis The NanoDrop Ultra FL and NanoDrop UltraC FL instruments are capable of both UV-Vis spectroscopy and fluorometry. The capable NanoDrop Ultra dsDNA (BR/HS) and RNA (HS) Fluorescence Assays allow for a more sensitive quantification of dsDNA and RNA and can detect down to 0.2 ng/µL dsDNA.
  • Wide spectral range Measure the full UV-Vis spectrum from 190–850 nm, allowing a range of assays as shown in the table below
  • Routine and custom embedded applications DNA, RNA, and protein quantification, full spectral data, purity ratios (A260/A280, A260/A230), and more
  • Local or PC control software Operate your instrument from the glove-compatible touchscreen or your USB-connected PC, including custom methods and data export capabilities
  • Integrated qPCR Recipe Calculator Fully integrated qPCR recipe calculator to save time, optimize efficiency, and achieve reliable downstream results with precise reaction setup.
  • Elevated Sample Insight Software with the ability to differentiate between DNA and RNA for samples of mammalian, plant, and bacteria origin.
Sample or analysis type Technique (Wavelength)
Peptides Absorbance (205 nm)
DNA and RNA Absorbance (260 nm) and Fluorescence
Purified protein Absorbance (280 nm)
Toxicology assays and industrial dyes Absorbance (490 nm)
Gold nanoparticles Absorbance (520 nm)
Colorimetric protein assay: BCA Absorbance (562 nm)
Colorimetric protein assay: Bradford Absorbance (595 nm)
Colorimetric protein assay: Modified Lowry Absorbance (650 nm)
Colorimetric protein assay: Pierce 660 Absorbance (660 nm)
Optical density measurements Absorbance (600 nm)

Powerful, flexible UV-Vis spectrophotometer and fluorometer operating and compliance software

NanoDrop One Spectrophotometer offers one-touch access to applications, even with gloved hands
 

The high-definition, glove-compatible touchscreen interface of NanoDrop Ultra spectrophotometers and fluorometers give you complete onboard control of your instrument. The user-friendly interface includes stom methods, automated software update notifications, data export to Cloud capabilities, and many other great features. An integrated, onboard Learning Center maintains an archive of technical support documents and troubleshooting tips to help guide and inform you when working with more challenging samples.

In addition to the onboard touchscreen interface, free NanoDrop Ultra PC Control software allows user control of the instrument from a USB-connected PC.

For regulated labs, optional SciVault 2 Software for NanoDrop Ultra allows instrument use in a 21 CFR Part 11 compliant

 environment. This module, which no longer requires the use of a connected computer, helps to maintain user roles, the records required for user account access, audit trails, electronic signatures, automated data back-up, and more.

Onboard touchscreen interface. The ergonomic, standalone NanoDrop Ultra instruments offer onboard control via a high-definition, glove-compatible touchscreen.  

Integrated qPCR recipe calculator

Obtaining a sample concentration is just the beginning – calculating how much sample goes into your downstream reaction can be tedious. Use the built-in library of kits, or add your favorite, to let the software run the math for you. The qPCR recipe calculator suggest how to prepare the reaction based on your sample’s concentration.

Acclaro Sample Intelligence Technology

Thermo Scientific Acclaro Sample Intelligence Technology software (included with NanoDrop Ultra spectrophotometers and fluorometers and NanoDrop Eight spectrophotometers) analyzes absorbance across the whole UV-Vis spectrum, not just wavelengths specific to nucleic acids and proteins. It then applies powerful chemometric algorithms to find and correct for contamination in your samples. You get more reliable downstream assay results and avoid potential rework with this efficient, breakthrough capability.
  • Next-generation quality assessment using Acclaro software Chemometric algorithms allow NanoDrop instruments to identify and correct for specific contaminants, taking quality assessment to the next level.
Acclaro Sample Intelligence software can even use full-spectrum data to tease apart DNA from copurified RNA and calculate corrected DNA concentrations for samples of mammalian, plant, and bacteria origin.
  • Verify sample quality in seconds Identify sample contaminants and obtain corrected concentration results
  • Identify contaminants that absorb in the analysis region Determine absorbances from known contaminants like protein or phenol in dsDNA or RNA samples or nucleic acids or phenol in protein samples
  • Rely on accurate results Help ensure measurement integrity with embedded sensor and digital image analysis
  • Access information through alerts Receive instant feedback about sample quality with on-demand technical support and guided troubleshooting
  • Analyze the entire spectrum to expose other contaminants Detect the presence of guanidine HCl and guanidinium isothiocyanate in nucleic acid samples, dsDNA in RNA, or RNA in dsDNA

Advanced connectivity for data storage, printing, and sharing

Improve your laboratory’s productivity and expand your collaborations with connected laboratory instruments that accelerate your research. Features include: NanoDrop One offers advanced connectivity to improve productivity and expand collaborations with connected laboratory instruments
  • Export to the cloud – Export data directly to popular cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
  • Direct LIMS integration – Send data directly into your LIMS with the assistance of an integrated RESTful API.
  • View data your way – Customize and preview the information included in the data file prior to export to ensure it meets any downstream needs. – Export data directly to popular cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
  • Direct LIMS integration – Send data directly into your LIMS with the assistance of an integrated RESTful API.
  • View data your way – Customize and preview the information included in the data file prior to export to ensure it meets any downstream needs.
Or, when you want to work at your desk, you can use NanoDrop Ultra PC Control software to view and analyze your data or explore Acclaro support features on your PC. Then, when your results are ready, you can print directly or seamlessly transfer results to a PC or a connected Cloud platform using a USB, *Wi-Fi, or Ethernet connection.

Choose the pedestal-only or pedestal/cuvette option

NanoDrop OneC instrument offers built-in cuvette capability
Although all models in the NanoDrop Ultra family have the pioneering microvolume sample retention capability of our pedestal, the NanoDrop UltraC and NanoDrop UltraC FL instruments include a built-in cuvette capability. The cuvette position adds experimental flexibility and increases the dynamic range. Combining the pioneering sample-retention system with cuvette capability accommodates both low and high concentrations (0.2–27,500 ng/µL dsDNA and 0.006–820 mg/mL BSA).   Use cuvettes to measure dilute samples and optical density of bacterial cultures or to perform kinetics experiments. The cuvette position can be used with the arm up or down and includes temperature control and stirring features.

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