CRYO ARM™ 300 II is a cryo-electron microscope that specializes in the observation of electron beam-sensitive specimens, such as protein, for single particle analysis, tomography and MicroED. This system offers improved stability, throughput and ease of use compared to the previous generation of cryo-EMs. Moreover, this is an all-in-one system that can handle everything from screening to data acquisition, allowing for more flexibility in operation at the customer sites to meet the needs of the facility. These improvements allow users to obtain high quality images by simple operations even for those who have never used an electron microscope before.
Main Features
- High-speed imaging achieved by optimal electron beam control
To support the complete workflow from sample screening to image data acquisition, it is of prime importance to improve throughput for image data acquisition. In the CRYO ARM™ 300 II, precise movement of the specimen stage is combined with excellent beam-shift performance for high-speed data acquisition. In addition, a unique “Koehler mode” illumination allows for uniform beam illumination onto a specific site on the sample, enabling more images to be acquired from a smaller area. These new technologies enable the CRYO ARM™ 300 II to deliver two times or higher throughput than the CRYO ARM™ 300. - Improved hardware stability for high-quality image acquisition
In performing SPA, although acquisition of a great number of images improves throughput, this is not enough. High-resolution data reconstruction from a small number of images is required, and this is achieved by high image quality. For this objective, the CRYO ARM™ 300 II is equipped with a new cold field emission gun (Cold FEG). This FEG has previously been incorporated into the GRAND ARM™ 2, a high-end atomic resolution analytical electron microscope released in 2020. Like the case of the GRAND ARM™ 2, this new Cold FEG produces a highly stable probe current. The CRYO ARM™ 300 II is also equipped with a new in-column Omega energy filter which has excellent stability. This new Cold FEG and new Omega energy filter enable users to acquire superbly high signal-to-noise ratio images. - Higher operability through system improvement
The CRYO ARM™ 300 II includes various system improvements. The microscope is equipped with the new JADAS (JEOL Automated Data Acquisition System for Cryo-EM) software (upgraded to version 4) for performing SPA. This JADAS 4 software, developed for novice users, provides improved operability for data acquisition. The new Omega filter incorporates an automatic self-adjustment system for reducing routine maintenance.
The specimen stage of the microscope has excellent positional reproducibility. Even if the user transfers samples back and forth between the microscope column and sample storage, an initial low magnification image of the whole sample grid (global map) can still be used. It is also possible to stop image data acquisition and rapidly screen sample grids during this short stop of data acquisition.
The automated specimen exchange system features storage of up to 12 samples. Sample grids can be kept clean in storage for weeks or longer without ice contamination of the samples.
Quick Collection
The JEM-3300 CRYO ARM™ 300 II achieves high speed data acquisition by combining beam shift with precise specimen stage movement.
The excellent beam control of this system minimizes the beam tilt that causes coma aberration, even with beam shift.
Hence, the throughput can be increased without degrading image quality.
Zero Fringe System
In addition to the ordinary beam illumination, the CRYO ARM™ 300 II has a unique “Koehler mode” illumination.
This mode has no interference fringes and eliminates electron-beam damage to areas not used for imaging and allows you to get more images from a smaller area.
Highly Flexible Operation
－As you would like－
High precision stage drive
The CRYO ARM™ 300 II has a rotation free stage. The advantage of this stage is excellent positional reproducibility. Even if you move samples back and forth between the microscope column and sample storage, you can reuse low magnification images, which we call “global map”. It allows for obtaining good sample conditions to capture high-quality data because exposure to the electron beam is greatly reduced.
JADAS 4
The CRYO ARM™ 300 II is equipped with new version of “JADAS*” software to enable novice users to acquire data.
New JADAS allows the users to automatically acquire image data by simply selecting the acquisition parameters with a wizard.
* JEOL Automated Data Acquisition System
Contamination free sample storage and removal
The grids can be kept clean in storage for weeks or longer in the CRYO ARM™ 300 II.
Moreover, those grids can be replaced from a single grid by the transfer magazine. Liquid nitrogen temperatures and nitrogen gas atmosphere are kept at specimens ejection.
High Stability
－Any time you get excellent images－
New Cold Field Emission Electron Gun
The CRYO ARM™ 300 II is equipped with the new cold field emission electron gun (CFEG).
This new gun not only has a low energy spread, but also provides an improved probe current stability, allowing users to obtain high quality images at any time.
New Omega filter
The CRYO ARM™ 300 II is equipped with a new improved in-column energy filter, the Omega filter, which is self-adjusting and always delivers the best performance with high stability.
