Sophisticated cell analysis doesn’t have to be complicated or costly.
The Guava® Muse® Cell Analyzer is an easy to use, simple, compact, robust instrument for your cell analysis needs.
With the Guava® Muse® Cell Analyzer, you can now achieve highly quantitative results at a fraction of the price, effort, and time. The Muse Cell Analyzer packs 3-parameter analysis into a compact, easy to use benchtop device, making flow cytometry accessible to anyone, anytime. A user-friendly touchscreen interface, intuitive cell analysis software, and optimized assays work to simplify your research.
Features of the Guava Muse:
- Quantitative data at the single-cell level
- Simple and effortless operation
- Intuitive cell analysis software and touchscreen user interface
- Rapid setup and analysis
- Optimized Muse assays
- Compact size: footprint of only 8 in x 10 in (20 cm x 25 cm)
- Flow cytometry technology in an affordable instrument
The Muse Instrument has an integrated touchscreen and software for data acquisition and analysis using optimized Muse Assays. Its microcapillary flow cell is engineered for acquisition of both suspension and adherent cells 2-60 microns in diameter.
How the Muse Cell Analyzer Works
Muse Cell Analysis Principle
The Muse Cell Analyzer uses miniaturized fluorescence detection and microcapillary cytometry to deliver single-cell analysis, which is highly quantitative — especially when compared with traditional bulk methods like microscopy and Western blot.
Sample collection, acquisition, and analysis of results is achieved in just a few steps. The user interface is designed to be intuitive, so you spend less time with experimental setup and analysis for the critical cell health assays you need most.
Laser-based Fluorescence Detection
The system delivers high-performance cell analysis using a microcapillary technology and miniaturized optics which occupy one-tenth the space of a typical cytometer. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters. As a result, Muse provides more quantitative results than non-flow based imaging systems, which only examine up to two parameters, are time-consuming, and provide less quantitative data.
Novel, Miniaturized Flow Technology
The Muse Cell Analyzer uses miniaturized fluorescent detection and microcapillary technology to deliver truly accurate, precise, quantitative cell analysis compared to other methods. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters – cell size (forward scatter) and 2 colors (detected in the red and/or yellow channels). The system uses a microcapillary and miniaturized optics, which occupy one-tenth the space of a typical cytometer. This means that the instrument occupies only an 8 in x 10 in (20 cm x 25 cm) footprint. A green diode laser is used for excitation, and a uniquely designed series of retro-reflective lenses provide maximum light capture and sensitivity.
Highly Intuitive Touchscreen Interface
Muse features a highly intuitive touchscreen interface that allows simple step-by-step operation, so easy that no flow expertise is required to run assays. The touchscreen prompts you through simple on-screen instructions and guides you though sample loading to simple setting adjustments to results—in just a few steps.
Software
Muse Software is designed to be simple enough for any user to follow setup instructions to achieve sample acquisition, on the first use. Assay-specific software modules include sample graphs to make population gating setup foolproof. Results are displayed in both graphical and statistical formats specific to each application, making analysis unambiguous.
Muse Cell Analyzer Reported Statistics on Viable Cells
The figure below shows the data output for the Muse Count & Viability Assay. Healthy Jurkat cells were mixed with heat-killed Jurkat cells and stained with Muse Count & Viability Reagent, and then analyzed on the Muse Cell Analyzer. Data output include summary data (not shown) and optional dot plots (shown here). Reported statistics include viable cells/mL, % viability, and the total cells/mL. The left hand dotplot shows viability vs. cell size; the right hand plot shows viability vs. nucleated cells.
Muse Cell Analyzer Performance
Cell quantitation and analysis techniques, such as hemocytometry, that are based on image analysis have limited sensitivity and objectivity, and sample sizes may be too small for statistical analysis. Muse assays use relative fluorescence and flow cytometry to assess thousands of cells per sample, rendering data that is more accurate, more precise, and more reliable than traditional methods.
Accurate Cell Concentrations
The Muse Cell Analyzer counts cells more accurately than manual hemocytometry or image-based automated analysis.
Multiple adherent and suspension cell types were harvested, diluted, and counted. Cell counts from all three methods were averaged to obtain “theoretical cell concentration”. Each point represents the average cell concentration of 3 replicates, and each data series was fit with linear regression. Muse cell analysis data was correlated with the theoretical concentration with slope closest to 1, indicating superior accuracy.
Precise Results
The Muse Cell Analyzer counts cells and measures viability more precisely with smaller coefficients of variation (%CV) than manual hemocytometry or image-based automated analysis. When compared with image-based automated counting methods and manual hemocytometers, the Muse Cell Analyzer exhibited a narrower range of %CVs and consistently provided variation of less than 10% over the entire range of samples tested. Higher %CVs were observed for the Trypan blue-based methods, particularly at lower cell concentrations.
| Analysis | Cell Concentration | Viability | ||
| Average % CV | % CV Range | Average % CV | % CV Range | |
| Image-based Automated Counter | 9.2% | 1.2-23.3% | 3.7% | 0.8-12.1% |
| Manual Hemocytometer | 9.2% | 6.3% | 4.5% | 0.5-9.2% |
| Muse | 4.0% | 0.3-8.8% | 2.2% | 0.4-5.6% |
The Muse Cell Analyzer provides superior precision for cell concentration and viability measurements, compared to Trypan blue-based analyses. Data are based on triplicate measurements of 30 cellular samples from suspension and adherent cell lines at multiple concentrations and viabilities.
Linear Results Across Multiple Cell Types
The Muse Cell Analyzer performs with exceptional accuracy across multiple cell lines and a wide sample concentration range.
The concentration measurements for serial dilutions of five representative cell lines are shown, and include both adherent and suspension cells. Each point represents the average of three replicates. Expected cell concentrations were calculated by measuring the stock sample concentration with the Muse Cell Analyzer, then dividing by the dilution factor to obtain theoretical concentrations of diluted samples. Data show the comparison of observed vs. expected cell concentrations.
